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KMID : 0378019710140040079
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New Medical Journal
1971 Volume.14 No. 4 p.79 ~ p.83
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Histochemical Study on Protein of Human Fetus Tooth Germ with Amelogenesis and Dentinogenesis
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Abstract
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It has been suggested that several organic components have important roles in the formation and the mineralization of dental hard tissue matrices.
Many studies on the distribution of proteins were carried out, but the results were different. So this investigation dealed with the localization of the proteins (amino acids) in the developing tooth germ, and correlated their appearances in terms of chronology.
The developing tooth germs were removed from the upper and lower deciduous molar regions of 6 human fetuses, ranging from 14 to 22 weeks of age. They were fixed in 10% formalin, absolute alcohol and Warren et McManus´ solution, and, without decalcifying, embedded in paraffin of vaccum bottle.
The serial sections were histochemically stained by alloxan-Schiff reaction (a-amino acid groups), Pearse´s method (-SH and -SS groups), Millon reaction of Bensley et Gersh (phenolic groups), xanthoprotein reaction (phenyl groups) and Warren et McManus´ method (guanido groups).
The comparative staining methods were PAS reaction, Lillie´s basophilia and hematoxwlin eosin stain.
The results were as follows:
1) a-Amino acid, -SH and phenolic group reactions had marked activities in the preenamel, but -SS and phenyl groups had lesser activities in the preenamel than in the ameloblasts.
2) a-Amino acid and phenyl group reactions had marked activities in the predentin, but -SS groups had lesser activities in the predentin than in the odontoblasts.
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